Recombinant Mouse IL13RA2/CD213A2 Protein (His Tag)(Active) Size: 100μg Catalogue Number: PKSM040949-100 Citations, Manuals and MSDS Available upon request. Abbreviation: IL13RA2; CD213A2 Target Symptom: CD213a2 Target Species: Mouse Expression Host: HEK293 Cells Application: Cell culture Fusion Tag: C-His Accession: NP_032382.1 Background: Interleukin-13 receptor subunit alpha-2 (IL13RA2/IL-13RA2) is also known as also known as cluster of differentiation 213A2 (CD213A2), IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, and IL-13RA2. The IL13RA2 is often overexpressed in brain tumors, making Il13ra2 one of the vaccine targets for immunotherapy of glioma. IL13RA2/IL-13RA2 is a cancer-associated receptor that is present in greater than 80% of High Grade Astrocytomas (HGA) and has recently been recognized as a cytokine that predisposes breast cancer cells to metastasize. Expression of IL13Rα 2 was rapidly lost from the surface of transduced cells grown in culture. The loss appeared to be related to ligands present in fetal bovine serum in the medium. None of the malignant glioma cell lines cultivated in vitro and tested to date exhibited the IL13Rα 2 receptor. A recombinant virus (R5111) enters cells via its interaction with the IL13Rα 2 receptor in a manner that cannot be differentiated from the interaction of wild-type virus with its receptors. Activity: Measured by its ability to inhibit IL13-dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 6-24 μg/mL. Sequence: Met 1-Lys 334 Purity: > 98 % as determined by reducing SDS-PAGE. Formulation: Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. Reconstitution: Please refer to the printed manual for detailed information. Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method. Calculated MW: 38 kDa Observed MW: 45-50 kDa Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. Shipping: This product is provided as lyophilized powder which is shipped with ice packs. Research Use Only